Acetic acid fermentation and electricity production A. aceti was inoculated
into the cultivation medium, and the culture was incubated at 30C for 72 h,
agitated by shaking at 150 strokes/min. The cultivated A. aceti cells were collected by
centrifugation for 10 min at 2900 g and then washed with distilled water twice. A
sample of the collected cells, possessing a wet cell weight of 0.2 g, was suspended in
50 ml of the fermentation medium, which contained 1% (w/v) peptone, 1% meat
extract and 0.5% ethanol. The resulting A. aceti cell suspension in the fermentation
medium, i.e., the fermentation suspension, was injected into the MFC anode
chamber through the hole, and the hole was then sealed to create an anaerobic
environment within the anode chamber. For repetitions of fermentation and the
production of electricity, the fermentation suspension was recovered from the MFC
anode chamber through the hole in the wall without disassembling the MFC setup.
The anode electrode was used without any surface cleaning for the following batch
fermentation. The A. aceti cells in the recovered fermentation suspension were
collected and resuspended in 50 ml of fresh fermentation medium. The new
fermentation suspension was then injected into the MFC anode chamber. All experiments
were performed at 30C with mild agitation by magnetic stirring. Conventional
aerated acetic acid fermentation was also carried out in a glass bottle
under identical conditions to those described above, with the exception of the
addition of aeration and the initial ethanol concentration (2%) that was used.
Ethanol and acetic acid were quantified by GC analysis, and the power generation
characteristics of the MFC were measured by electrochemical analysis.
Acetic acid fermentation and electricity production A. aceti was inoculatedinto the cultivation medium, and the culture was incubated at 30C for 72 h,agitated by shaking at 150 strokes/min. The cultivated A. aceti cells were collected bycentrifugation for 10 min at 2900 g and then washed with distilled water twice. Asample of the collected cells, possessing a wet cell weight of 0.2 g, was suspended in50 ml of the fermentation medium, which contained 1% (w/v) peptone, 1% meatextract and 0.5% ethanol. The resulting A. aceti cell suspension in the fermentationmedium, i.e., the fermentation suspension, was injected into the MFC anodechamber through the hole, and the hole was then sealed to create an anaerobicenvironment within the anode chamber. For repetitions of fermentation and theproduction of electricity, the fermentation suspension was recovered from the MFCanode chamber through the hole in the wall without disassembling the MFC setup.The anode electrode was used without any surface cleaning for the following batchfermentation. The A. aceti cells in the recovered fermentation suspension werecollected and resuspended in 50 ml of fresh fermentation medium. The newfermentation suspension was then injected into the MFC anode chamber. All experimentswere performed at 30C with mild agitation by magnetic stirring. Conventionalaerated acetic acid fermentation was also carried out in a glass bottleภายใต้เงื่อนไขเหมือนกับที่อธิบายไว้ข้างต้น ยกเว้นการนอกจากนี้ aeration และเอทานอลที่เริ่มต้นความเข้มข้น (2%) ที่ใช้เอทานอลและกรดอะซิติกได้ quantified โดยวิเคราะห์ GC พลังงานไฟฟ้าลักษณะของ MFC ถูกวัด โดยการวิเคราะห์ทางเคมีไฟฟ้า
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