Biogas fermentation proved that all substrates were characterized by good biogas production potential. Concentration of methane in each sample was higher than 50%. From all examined substrates, common reed reached the highest yield of biomethane production (108 m3 Mg−1 FM and 212.5 m3 Mg−1 DM). The lowest biomethane efficiency, presented as m3/Mg FM, was that of Jerusalem artichoke (66.0), while in units m3 Mg−1 DM it was that of willow (150.1). It has to be emphasized that willow is not commonly used as a substrate for biogas production because of its high lignocellulose content. However, in this research, the willow used was from the first year of cultivation, with a low lignin content.