3.3. Stimulating effect of GLP-1 analogue as part of a chimeric globulin protein on insulin secretion from MIN6 cells
We measured insulin secretion levels of 10 T1 grains of the 185-62401 line, the 185-70116 line and untransformed rice in a mouse pancreatic beta-cell line, MIN6 [13]. No biological activity of GLP-1 analogue was detected in grains of untransformed rice seeds. Meanwhile, the 185-62401 and 185-70116
lines showed biological activity in insulin secretion at levels of 989.4 and 734.3 ng/ml (Table 1). The biological activity of synthetic [Ser8, Gln26, Asp34]-GLP-1 was slightly less than that of native GLP-1 (7-36) amide (data not shown).