The mobile phase was 5 mM sulfuric acid, deionized
water filtered through 0.22 lm filters. Operating conditions for
the HPLC column were 60 C with a mobile phase flow rate of
0.6 mL/min. For sample analysis, 10 lL of sample was injected
and complete sample elution could be accomplished within
48 min. An external standard consisting of glucose, xylose, acetic
acid, HMF and furfural was used for quantification.