The extracts were evaporated(ระเหย) resulting(ส่งผลให้) aqueous(เกี่ยวกับน้ำ,น้ำ,องค์ประกอบน้ำ) solutions(สารละลาย) were filtered(กรอง) and extracted(สกัด,แยกออก) three times with 10 ml diethyl ether. The pooled extracts(สารสกัด) were then loaded on a solid phase extraction cartridge (500 mg of sorbent, aminopropyl) and the cartridges were washed with 7.0 ml of trichloromethane and 2-propanol (2:1, v/v).