Temperature has a significant effect in the germination of spores which could determine the successful of the SSF system. Therefore, temperature become one of key parameters in SSF system (How and Ibrahim, 2004; Pang and Ibrahim, 2005). Fig. 3 shows the effect of cultivation temperature on mannanase production. The highest mannanase activity was achieved at the room temperature (28±2oC) with the value of 97.00 U/g substrate and the fungal growth of 1.44 mg glucosamine/g substrate.