The electrospinning process was car-ried out using a voltage of 25 kV, a needle tip-collector distance
of 20 cm, a solution feeding rate of 1 l min−1
, controlled with a
syringe pump (KDS, KD Scientific nc., USA) and a deposition time
of 60 min. During the electrospinning process, the environmental
conditions were controlled to be the following: a temperature in
the range 20–25
◦
C and a relative humidity in the range 45–50%.
In order to increase the stability in water, the prepared keratin
nanofibrous membranes were treated at 180
◦
C for 2 h