After that the injector heating was turned off and the temperature was decreased to 100 ºC for the next injection. This injector temperature program was used to improve the analyte transference to the column and to avoid thermolysis of sugars from the extracts. An injection volume of 1 µL (splitless) was used. After 2.0 min a split ratio of 20:1 was used to clean the injector for the next injection.