The transformation of organic matters in swine, cow and chicken manures was compared and evaluatedusing elemental analysis, FTIR,13C NMR, pyrolysis/GC/MS, Biolog and multiple fluorochrome over 60 dayscomposting. The results revealed that cow manure exhibited the greatest C/N and aromaticity, whereaschicken manure exhibited the highest nitrogen and sulfur contents. O-alkyl-C was predominant carbonstructure in the three manures. Alkyl-C and carboxyl-C were decomposed dramatically in initial 10 days,and mineralization of O-alkyl-C dominated the curing stage. During pyrolysis of chicken, cow, and swinemanures, the majority products were fatty acids, phenols and cholestene derivatives, respectively, how-ever, phenols and cholestene derivatives were strongly reduced in the mature manures. Furthermore,microorganisms in the raw cow, chicken and swine manure demonstrated the highest degradation capa-bilities for carbohydrates, lipids and amino acids, respectively. Spatial differences in the contents of solidorganics in the manure particles were negligible through detection by multiple staining methods duringcomposting