This study clearly indicated that the used nSSR
molecular markers are highly polymorphic and efficient
to discriminate among the 40 rootstocks belonging to eight
Citrus species. An important level of genetic diversity is
reported for the studied accessions, which highlights the
utility of Tunisian germplasm as genetic resources for
further Citrus breeding programs. Furthermore, the phylo-
genetic analysis of the studied sample confirmed previous
reports concerning the origin of the studied Citrus species.