A total of 87 strains of LAB and bifidobacteria were isolated. They were screened for antimicrobial activity against Staphylococcus aureus. Staphylococcus aureus is resistant to methyl silicone which is an antibiotic. The antimicrobial activity against Staphylococcus aureus using the cell – free supernatant (CFS) of LAB and bifidobacteria was detected