Results obtained for HPLC quantification of individual black
mulberry anthocyanins showed a decrease in anthocyanins from
fruit to juice (Table 3). These results do not align well with the
results of spectrophotometric TMA measurements, which showed
a significant increase (214%) as a result of juice processing (Table 1).
The spectrophotometric method clearly lacks specificity, which
makes it necessary to perform more specific methods to obtain
more accurate results, as was done in the work reported here.
Another finding was that the final black mulberry juice had substantially higher levels of phenolic acids (approximately 4-fold
higher) compared to the raw fruit (Table 3). This could possibly
be related with the thermal processes applied during food processing which are suggested to release more bound phenolic acids due
282 M. Tomas et al. / Food Chemistry 186 (2015) 277–284
to the breakdown of cellular constituents, making them more
accessible in the extraction process (Dewanto et al., 2002