Methods: The study was conducted during a period of 4 years. Medical and nursing staff
followed a problem-based education program on hand hygiene. Hand hygiene practices
before and after the education program were compared by guided observations. The
incidence of nosocomial infections in VLBW infants was compared. In addition, numbers of
nosocomial bloodstream infections per day-at-risk in very low birth weight infants were
analyzed by a segmented loglinear regression analysis.