Although no tendency was observed with the variation in moisture content in each blend (in terms of peak temperature or enthalpy values of these peaks, results not shown here), it seems that the greater the added CO level the lower the moisture content needed for this sub-Tg endothermic peak to appear.
Sub-Tg endotherms of glassy starches were firstly reported by Kalichevsky, Jaroszkiewicz, and Ablett (1992), occurring at around 50 ◦C for amorphous amylopectine samples with moisture content between 12 and 15%.