Pre-ovogenic female adult specimens of M. borellii of similar
size and weight (40 ± 5 mm and 1 ± 0.2 g) were collected in August
in a water course close to the Río de la Plata river, Argentina. Females
were taken to the laboratory and kept in dechlorinated tap
water at 20 ± 5 C, under a 14:10 L:D photoperiod with an formulated
diet (Collins and Petriella, 1999).