The fatty acid profile was determined by fatty acid
methyl ester (FAME)/gas chromatography using acetyl
chloride as a reagent for transesterification according
to the method of Christie (1993). The lipid sample
(5 mg) was treated with 1 ml methanol:benzene (3:2, v/
v) and 1 ml acetyl chloride: methanol (1:20, v/v). Then
50 ll magaric acid (0.5 lg/ll), used as internal standard,
were added