The use of the mixture of cellulase and pectinase at pH 4.5, at 28oC for 1 hr followed by α-amylase at pH 5.5, at 100oC for 2 hrs and finally glucoamylase at pH 4.5, at 60oC for 24 hrs was the optimum condition for enzymatic cassava waste pretreatment in this study and also gave the maximum reducing sugar at 6.2% (w/v).