High alkaline conditions could cause
undesirable side reactions and potential toxicity, such
as lysinoalanine, thus losing the nutritive values of
protein. In addition, high alkaline conditions could cause
the following: (1) denaturation and hydrolysis of proteins;
(2) increased Maillard reaction which causes darkcolored
products; (3) increased extraction of nonprotein
components which coprecipitate with protein and lower
the isolate quality (Kolar et al., 1985).