Total Viable Count was determined by pour plate method as described. One ml of the sample from the three dilutions (10-1, 10-2 and 10-3) were taken and added to each duplicate Petri dish. Plate Count Agar was added to each Petri dish and incubated at 35°C for 48±2 h, after incubation colonies were counted by Colony Counter and result was expressed as CFU/ml.