The embryogenic calli were subjected to gamma radiation
using Gamma Cell 220 at dose rate of 9.6 Gy
min-1. The irradiation doses were 10, 20, 30, 40, or 50
Gy. Radiation-treated calli were immediately cultured
on CIM to eliminate the radiolysis hazards and subcultured
at least thrice, at monthly intervals, on the same
medium (CIM) before using for further studies. Survival
percent of the calli was recorded in terms of white
proliferating clumps (WPCs).