Creatinine (Cr), uric (UA) and ascorbic acid (AA) are common constituents in human fluids. Their
abnormal concentrations in human fluids are associated with various diseases. Thus, apart from the
endogenous formation in human body, it is also important to examine their sources from food products.
In this study, a rapid and accurate HILIC method was developed for simultaneous determination of Cr, UA
and AA in bovine milk and orange juice. Milk samples were pretreated by protein precipitation, centrifugation
and filtration, followed by HPLC separation and quantification using a Waters Spherisorb
S5NH2 column. The developed method has been successfully applied to determine the concentration of
UA, AA and Cr in milk and fruit juice samples. The milk samples tested were found to contain UA and
creatinine in the concentration range of 24.1–86.0 and 5.07–11.2 lgmL1, respectively. The orange juices
contain AA over 212 lgmL1.