2.4. Hepatoprotective evaluation of TLE and SL against ethanol in primary cultures of rat hepatocyte
After 24 h of culturing, hepatocytes were treated with ethanol (96 μl/ml) and various concentration of TLE (2.5, 5.0, 7.5 and 10.0 mg/ml) or SL (1, 2 and 3 mg/ml) for 2 h. After 2 h incubation, cell viability was tested using MTT assay, ALT and AST release.
All experiments were performed at least three times using different cell preparations.