2.7. Animal model and histological analysis
were blocked with protein block, serum-free (Dakocytomation. Inc, US) for 1 h, and
treated with a primary antibody iNOS (Santa Cruz Biotechnology. Inc, USA) for 18 h
and a secondary antibody, mouse IgG labeled with Alexa Fluoro 555 (Invitrogen, US)
for 3 h. Cells were also stained with DAPI for nuclear counterstain.