Identification and quantification of IAA
Strain W19 was incubated in Landy medium containing
L-tryptophan to produce IAA. The IAA standard
chemical solution and culture filtrates were both
analyzed by HPLC. The retention time of the IAA was
10.46 min (Fig. 5). Three days after incubation, the
concentration of IAA was 7.35 mg L−1, calculated by
the regression equation.