The filtrate was collected for determining
protein leakage (Liu et al. 2010), and the mycelia were
used in the malondialdehyde (MDA) assay for lipid
peroxidation. Soluble protein content in the filtrate was
determined according to Bradford (1976) with bovine serum
albumin (Sigma-Aldrich, Germany) as a standard. Protein
leakage was expressed as mg g–1 fresh weight of mycelia.
For assaying lipid peroxidation, a method based on the
reaction of thiobarbituric acid with MDA derived from lipid
peroxidation was employed. Detection of thiobarbituric acid
species was carried out by a colorimetric assay (Ritter et al.
2008). The lipid peroxidation was expressed as μg g–1 fresh
weight of mycelia.