Nearly all well-established morphospecies in the subgenus Trichodagmia formed well-defined groups in the
Neighbour Joining (NJ) tree based on DNA barcodes (Fig. 1). Genetic divergence between morphospecies
averaged 11.2% (range 2.8–19.5%), whereas intraspecific genetic divergence within morphologically distinct
species averaged 0.5% (range 0–1.28%) (Table 1). In known species complexes, maximum values of genetic
divergence (3.28–3.79%) indicated the presence of cryptic diversity (Table 4), and these values agree with those
observed by Rivera and Currie (2009) for Nearctic Simuliidae, and those observed by Cywinska (2006) for
Nearctic mosquitoes. The existence of well defined groups in the NJ tree within S. payneiis concordant with its
putative status as a sibling species complex, and similarly well defined groups within the morphospecies S. piperi,
S. duodenicornium, S. canadense,S. rostratumand S. tarsatum indicated cryptic variation and the possible
existence of species complexes (especially S. tarsatum with high levels of within-species diversity).
Nearly all well-established morphospecies in the subgenus Trichodagmia formed well-defined groups in the
Neighbour Joining (NJ) tree based on DNA barcodes (Fig. 1). Genetic divergence between morphospecies
averaged 11.2% (range 2.8–19.5%), whereas intraspecific genetic divergence within morphologically distinct
species averaged 0.5% (range 0–1.28%) (Table 1). In known species complexes, maximum values of genetic
divergence (3.28–3.79%) indicated the presence of cryptic diversity (Table 4), and these values agree with those
observed by Rivera and Currie (2009) for Nearctic Simuliidae, and those observed by Cywinska (2006) for
Nearctic mosquitoes. The existence of well defined groups in the NJ tree within S. payneiis concordant with its
putative status as a sibling species complex, and similarly well defined groups within the morphospecies S. piperi,
S. duodenicornium, S. canadense,S. rostratumand S. tarsatum indicated cryptic variation and the possible
existence of species complexes (especially S. tarsatum with high levels of within-species diversity).
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