Agar diffusion was carried out with RPMI 1640agar supplemented with glucose 2%. The inoculum wasprepared using 24-hour plate cultures of Candida andCryptococcus species. The colonies were suspended in 0.85%saline and the turbidity was compared with the 0.5 McFarlandstandard, to produce a yeast suspension of 1x106 to 5x106cells/mL. The suspension was loaded on a sterile cotton swabthat was rotated several times and press firmly against the