Materials and methods
2.1. Preparation of the crude extract and fractions
The leaves of Carissa carandas were collected in May 2010
from Karachi, Pakistan. The plant was identified by Dr. Ghulam
Rasool and deposited (Voucher no. 86465) in the Herbarium of
Department of Botany, University of Karachi. Fresh leaves (5 kg)
of Carissa carandas were repeatedly extracted with methanol
at room temperature.