Abstract Two Gram-staining-positive, aerobic, motile,
endospore-forming, rod-shaped bacteria, designated strains
Y24
T
and H9
T
were isolated from cold spring and carrot
(Daucus L.) samples, respectively, in Xinjiang Uyghur
Autonomous Region, north-western China. The taxonomic
positions of the two new isolates were determined by using
a polyphasic approach. Phylogenetic analysis based on
16S rRNA gene sequences and DNA–DNA hybridizations
showed that strains Y24
T
and H9
T
were two different novel
species belonging to the genus Paenibacillus, with Paenibacillus
hunanensis
FeL05
T
as their closest relative. The
genomic DNA G + C contents of the two isolates Y24
and H9
T
were 48.1 and 46.6 mol %, respectively. The cell
wall peptidoglycan contained meso-diaminopimelic acid.
The predominant menaquinone was both as MK-7. The
major cellular fatty acids were anteiso-C
15:0
, C
, isoC
16:0
, anteiso-C
17:0
and iso-C
15:0
16:0
. The polar lipid profiles
T