The surface- sterilized egg masses were either used for in vitro assay of egg hatch or incubated in sterile water for 3–5 days at 25 °C using modified Baermann funnel method (Southey, 1986) to obtain J2s.
The surface- sterilized egg masses were either used for in vitro assay of egg hatch or incubated in sterile water for 3–5 days at 25 °C using modified Baermann funnel method (Southey, 1986) to obtain J2s.