The standard dilution-plating technique was used for
isolation of bacterial strains from surface soil samples
collected from Boryung city. Strain BR-9T was isolated on
R2A agar (Difco) at 25 uC and routinely cultivated on R2A
agar at 30 uC. The type strains of two species of the genus
Pedobacter were used as reference strains for DNA–DNA
hybridization, phenotypic characterization and fatty acid
analysis. Pedobacter insulae DS-139T was obtained in our
previous study (Yoon et al., 2007b) and Pedobacter
koreensis KCTC 12536T was obtained from the KCTC