Reactions were stopped by the addition of 1 M sodium carbonate, and the released p-nitrophenol was detected by spectrometry at 410 nm. One unit of enzyme activity was defined as the amount of enzyme that produced 1 mmol of pNP per minute.
Reactions were stopped by the addition of 1 M sodium carbonate, and the released p-nitrophenol was detected by spectrometry at 410 nm. One unit of enzyme activity was defined as the amount of enzyme that produced 1 mmol of pNP perminute.