2.5. Real sample analysis
Urine samples were obtained from several healthy volunteers
before and after the ingestion of a commercial tablet containing
the standard doses of NSAID. It should be highlighted that volunteers
provided their consent after appropriate information about
the study and ethical guidelines were accomplished during the
complete study. Under no circumstances have the authors forced
in any way NSAIDs intake during this study.
On the other hand, environmental waters were sampled at different
sites including Turia River, Albufera Lake, irrigation ditches,
and sewage near to the metropolitan area of Valencia (Spain). Samples
were collected in hermetic polypropylene flasks, and stored for
short-times at 4 ◦C in the fridge. Filtration of samples using cellulose
filters was performed prior to analysis to remove solid and
seaweeds dispersed in water.