Three attenuated Aeromonas hydrophila vaccines were developed from the virulent 2009 West Alabama
isolates through selection for resistance to both novobiocin and rifampicin. When channel catfish (Ictalurus
punctatus) were IP injected with 4
×
105 colony-forming unit (CFU) of the mutants, no fish died.
However, when the same age and size matched channel catfish were IP injected with similar amount
of their virulent parents, 80–100% fish died. Similarly, when Nile tilapia (Oreochromis niloticus) were IP
injected with 2
×
108 CFU of the mutants, no fish died. However, when Nile tilapia were IP injected with
similar amount of the mutants, all fish died. Vaccination of channel catfish with the mutants at dose of
4
×
105 CFU/fish offered 86–100% protection against their virulent parents at 14 days post vaccination
(dpv). Vaccination of Nile tilapia with the mutants at dose of 2
×
108 CFU/fish offered 100% protection
against their virulent parents at 14, 28, and 56 dpv. Agglutination assay results suggested that protection
elicited by the mutants was partially due to antibody-mediated immunity. Taken together, our results
suggest that the three attenuated vaccines might be used to protect channel catfish and Nile tilapia against
the highly virulent 2009 West Alabama isolates of A. hydrophila.
Three attenuated Aeromonas hydrophila vaccines were developed from the virulent 2009 West Alabamaisolates through selection for resistance to both novobiocin and rifampicin. When channel catfish (Ictaluruspunctatus) were IP injected with 4×105 colony-forming unit (CFU) of the mutants, no fish died.However, when the same age and size matched channel catfish were IP injected with similar amountof their virulent parents, 80–100% fish died. Similarly, when Nile tilapia (Oreochromis niloticus) were IPinjected with 2×108 CFU of the mutants, no fish died. However, when Nile tilapia were IP injected withsimilar amount of the mutants, all fish died. Vaccination of channel catfish with the mutants at dose of4×105 CFU/fish offered 86–100% protection against their virulent parents at 14 days post vaccination(dpv). Vaccination of Nile tilapia with the mutants at dose of 2×108 CFU/fish offered 100% protectionagainst their virulent parents at 14, 28, and 56 dpv. Agglutination assay results suggested that protectionelicited by the mutants was partially due to antibody-mediated immunity. Taken together, our resultssuggest that the three attenuated vaccines might be used to protect channel catfish and Nile tilapia againstthe highly virulent 2009 West Alabama isolates of A. hydrophila.
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