The absorbance was
monitored at 280 nm. Peak identification was obtained by
spiking samples with inulin standard, which was acid and
heat hydrolyzed in a way similar to samples. Spectra of each
peak eluted from the column were also obtained for peak
identification and to check purity of peaks. Figure 1 indicates
a sample chromatogram of an acid-hydrolyzed cake containing
5% JAP. For the quantification of inulin in either JAP or
cakes, the sum of areas of all three peaks identified in Fig. 1
was used because the injection of pure commercial inulin
standard after acid hydrolysis indicated all three peaks.Recoveries
were higher than 87%.