For quantification of Salmonella in the tonsils (tonsilla
veli palatini), the tissue samples were flushed 3
times with sterile NaCl solution (0.85%) and minced
with scissors; 3 g of the samples were then placed into
a 50-mL plastic tube and diluted 1:10 in peptone water
(1% peptone) and homogenized with an Ultra Turrax.
The quantitative and qualitative detection of Salmonella
was performed as described for the fecal samples.