The obtained concentrations of amantadine in plasma samples of female and male volunteers were 126 ±11 and 110 ±9 ng mL-1 ,respectively. Also the obtained concentrations of amantadine in urine samples of female and male volunteers were 46 ±4 and 42 ±4 ng mL-1 ,respectively. In all cases three determinations (n=3) was performed on samples using standard addition method. The proposed procedure is so sensitive that drug content of plasma and urine was still detectable even after 12 or 24 hr