Bacterial spores represent a challenge for high pressure
technology and more information about their resistance is
required. Microbial spores suspended in foods or laboratory
model system could be inactivated by high pressure treatment
but compared to vegetative cells the treatment conditions
must be extreme: higher pressure and long exposure time at
elevated temperature (28). When pressure-temperature is
combined at 690 MPa and 80oC for 20 minutes, the treatments
was effective with a significant reduction in the Clostridium
sporogenes spore count (13). Successful treatment of Bacillus
stearothermophilus is observed were pressure treatments
is combined with moderate temperature (70oC) (22). There
are no published reports on the high-pressure resistance of
Clostridium botulinum spores, and their ability to withstand
high pressure at low or high temperatures is unknown (35).