Meat subsamples (25 g) were homogenized in 225 mL of peptone
saline (0.85% NaCl and 0.1% peptone in distilled water) for 30 s in a
blender. Twenty milliliters of the homogenate was centrifuged at a
speed of 10,000 ×g for 10min (Avanti J-HC, Berkman, USA).