Enzyme pre-treatment Initial studies were conducted to
optimize the incubation time and concentration of enzyme,
by treating 100 g of the powered samples of black pepper
and cardamom with different concentrations of the
enzymes for different incubation time (data not shown).
Briefly, well mixed aqueous solution of the enzyme
mixture of varying concentration was sprayed on to 100 g
of powdered spice material and the pH of the material was
adjusted to 5-5.5 with citric acid in water.