Ten milliliters of this suspension was gelatinized by placing a test tube of the sample in
boiling water for 10 min and autoclaving (120° C) for 10 min. After the suspension had cooled to 550C in an oven, the starch was further dispersed by an ultrasonic processor (Vibra-cell model
VC 40; Sonics & Materials, Danbury, CT) equipped with a 3-mm microtip, for 20 sec.