All participants underwent a complete physical examination and provided
fasting blood samples at baseline and at the completion of 12 wk of intervention
for measurement of several parameters.
Serum TG, TC, and HDL-C levels were measured by enzymatic colorimetric
kits (Spinreact, Spain). Plasma glucose was measured using glucose oxidase/
peroxidase (Lakeside, Mexico City). N ε -(Carboxymethyl)Lysine (CML) and
methylglyoxal (MG) derivatives in serum were quantified by enzyme-linked
immunosorbent assays (ELISAs), using two non–cross-reactive monoclonal
antibodies (mAbs; 4 G9 and MG3 D11 mAbs) raised against synthetic standards,
CML-bovine serum albumin (BSA) and MG-BSA, respectively. CML and MG were
measured by ELISA as previously described [25]. Test sensitivity for CML and MG
was 0.1 U/mL and 0.004 nmol/mL, respectively; the intra-assay variation was
?2.6% (for CML) and ?2.8% (for MG), and the inter-assay variation was ?4.1%
(CML) and ?5.2% (MG).
The researchers involved in the assessment of metabolic parameters and
biochemical determinations were unaware of the group assignment of
participants.