In our unpublished data, the highest frequency of piglets per SCNT embryo transferred was 12.5%. In addition, we could produce cloned piglets from transferring as few as 28 SCNT blastocysts.
This is a significant achievement, considering multiple embryos are needed to provide recognition signals to maintain the pregnancy. Thus, the culture of SCNT embryos is being improved, and the quality of the produced embryos is suitable for blastocyst transfers; nevertheless, there is still room for improvement.