Principles of the Procedure
Enzymatic Digest of Soybean Meal provides nitrogen and vitamins required for organism growth. Dextrose is included as
an energy source. Phenol Red is the pH indicator used to detect acid production. Cycloheximide inhibits most saprophytic
molds. The supplements, Gentamicin and Chlortetracycline, aid in selectivity of Dermatophyte Test Medium. Gentamicin
inhibits Gram-negative bacteria including Pseudomonas spp. Chlortetracycline is a broad-spectrum antibiotic, inhibiting a
wide range of Gram-positive and Gram-negative bacteria. Agar is the solidifying agent.