were higher at lower incubation temperatures. The percentage of the most abundant PUFA, 22:6(n-3), was highest at 1.8 °C compared to 4.9 °C and 8.0 °C (Table 4). This temperature effect also occurred in developing goldfish raised at 13 °C compared to 22 °C, and in adult fish (Greene and Selivonchick, 1987; Wiegand et al., 1991).