Fagaceae is popular plant for Forest restoration (Muñiz-Castro et al., 2015). The problem of Forest restoration is analogous seedling. Bar-HRM analysis is rapid detection and low cost so we choose Bar-HRM technique to classify Fagaceae. rbcL have property that High discriminating power and popular to use divide species in land plant (Janzen, D. H., 2009) so we select rbcL to design primer with plant model