Degeneration of
these cells by calcium chloride treatment could be a
possible cause of the lower content of glutathione in the
treated testis. The observed decreases in the levels of
enzymatic and non-enzymatic antioxidants are consistent
with our past studies [2,3,16,17]. In the present
study, the decreased level of GSH induced by calcium
chloride can seriously impair the optimal functioning of
these various catalytic activities of GSH dependent
enzymes [23]. Moreover, the elevated level of ROS can
cause oxidation of the mitochondrial pore and thereby
disrupt the mitochondrial membrane potential (Δψm)