The bags were subsequently kept in spawn running room with 75 -85% relative humidity until the formation of primordia. Then the bags were uncapped and transferred to the fruiting room to allow the normal development of fruit bodies. The bags were kept in bed position on rack (240 cm × 90 cm) at 25°C with a 12 hours photoperiod and 80–90% relative humidity. Adequate ventilation was provided to prevent increase of carbon dioxide gas, CO 2 concentration in the room which will inhibit the fruit body development (Norouzi et al., 2008)