FIGURE 3 Top: this 6-tzm frozen section was incubated in a conjugate made from an antiserum against rat
VLDL. Specific reaction product occurs in the same locations as in Fig. 2 and is present in all hepatocytes.
• 55. Bottom: this 6-v.m frozen section was incubated in a solution of Worthington peroxidase 5 mg/ml in
0.135 M phosphate buffer pH 7.4. There is no specific reaction product within the hepatocytes. Kupffer
cells in the sinusoids (arrows) contain reaction product due to endogenous peroxidase. • 55.