Microbial diversity of rumen has been predominately
studied by PCR amplification of 16S/18S rRNA genes
from community DNA followed by construction of
gene libraries, sequencing of selected clones, and phylogenetic
analysis using bioinformatics tools. Based on
the number of unique clones or operational taxonomic
units (OTUs) as well as their relative frequencies, the
presence and abundance of microbial genera/species
(Tables 2, 3 and 4) in rumen is established (Skillman
et al. 2004, 2006; McSweeney et al. 2007; Janssen and
Kirs 2008; Lozupone and Knight 2008; Zhou et al.
2011; Chaudhary et al. 2012). mcrA-gene-based rumen
diversity analysis of methanogens was performed in
cattle (Tatsuoka et al. 2004; Denman et al. 2007),
Microbial diversity of rumen has been predominatelystudied by PCR amplification of 16S/18S rRNA genesfrom community DNA followed by construction ofgene libraries, sequencing of selected clones, and phylogeneticanalysis using bioinformatics tools. Based onthe number of unique clones or operational taxonomicunits (OTUs) as well as their relative frequencies, thepresence and abundance of microbial genera/species(Tables 2, 3 and 4) in rumen is established (Skillmanet al. 2004, 2006; McSweeney et al. 2007; Janssen andKirs 2008; Lozupone and Knight 2008; Zhou et al.2011; Chaudhary et al. 2012). mcrA-gene-based rumendiversity analysis of methanogens was performed incattle (Tatsuoka et al. 2004; Denman et al. 2007),
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